Coastal Zones

Biography

Biography: Delianis Pringgenies

Abstract

Waste of mangrove is a collection of mangrove waste consisting of leaves, twigs, branches fall to the ground and eventually turned into decomposition. The research objective was to determine the types of bacterial symbionts of waste mangrove potential as an anti-bacterial, anti-bacterial bacteria subsequently used as bio-activator for the production of compost that contains nutrients. Research includes: isolation of bacterial symbionts, screening bacterial symbionts that potential as an anti-bacterial, DNA extraction using the High Pure PCR Template Preparation Kit (Roche), DNA amplification by PCR of 16S rDNA, DNA sequencing. Results 16S r-DNA sequences were analyzed and edited using Genetix program and sequence analysis of 16S rDNA. Results 16S r-DNA sequences were analyzed and edited using Genetix program and sequence analysis of 16S rDNA. Bacteria that potential as an antibacterial used as a bio-activator production and the production of bio-activator tested nutrient content of compost seaweed Sargasum. The results were compared with the activator products found on the market. The identification results are found there are 4 types of bacterial symbionts that potential as an anti-bacterial: the bacterium Pseudomonas sp., Flavobacterium sp., Acinetobacter sp., Bacillus subtilis. Production of bio-activator bacterial symbionts waste mangrove have better quality than the quality of bio-activator that exist in the market and liquid organic fertilizer without a bio-activator. Production of bio-activator bacterial symbionts waste mangrove have better quality than the quality of bio-activator that exist in the market and liquid organic fertilizer without a bio-activator. The conclusion is that the production of bio-activator of bacterial symbionts mangrove waste of potential as probiotics production environment.