Coastal Zones

Biography

Biography: BENHAR SAID

Abstract

The validation of an analytical method is defined in the ISO / IEC 17025 [1] standard as the confirmation by examination and provision of objective evidence that the particular requirements for a specific intended use are fulfilled. Intra-laboratory validation of an analytical method is defined as the act of submitting a method of analysis to a statistical study intra- laboratory based on a standardized and / or recognized protocol, and demonstrating that its scope, method of analysis satisfies predetermined performance criteria. Method validation is a primary requirement of the technical part of the ISO17025 standard [1]. In this work, we used the protocol DR-12-VMC Revision: 2009[2] on the validation of an analytical chemistry, for validation and to demonstrate that the method of dosage of Lead (Pb) in marine organisms , used internally, according to AOAC Official Method 999.10 by atomic absorption spectrophotometry with graphite furnace (quantitative method)[3] is suitable for the intended use. Also, through this study , we conducted to characterize the performance of the analytical method used , evaluating , through experimental work and statistical tests several parameters among the most often recommended are: the detection limit the limit "of quantification , the ratio of compliance, linearity, accuracy , sensitivity, specificity, accuracy , and finally recovery